Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LPMEELSVQG DLTYTEYPIK ILDTLTRVTR NKVIKMCKVQ WSHHGENEAT
 WEREEELRID FPHLFPRSSI SRTRLFLRGV GFVTPTLFAR NNTNREIISP
 YVDLTFMHHP MTPYSKANNK NICHQNYASC WILCLWSVFC DYTKKYININ
 KENTLIPREF KSRGLKYGEK ERVNINNIYV IILLRLVLFI SQYKLRINWH
 KIIIINGIKL KGKGKHKKKK 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          220 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 175 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 182   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   0.97
     Net Charge of CR: -3
     Discriminant Score:    -12.17
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -7.25
     Possible cleavage site: 31
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -4.46  threshold: -2.0
     INTEGRAL    Likelihood = -4.46   Transmembrane  175 - 191 ( 173 - 195)
     PERIPHERAL  Likelihood =  4.61
     modified ALOM score:   0.99
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 174 (174 Residues)
( 3) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 3
     Uncleavable? Ipos set to: 13
Discrimination of mitochondrial target seq.: 
     negative (-6.15)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.83 at (51)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  1.94
Chloroplast protein?  Status: negative (-10.02)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 79%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.198
SKL motif (signal for peroxisomal protein): 
     pos: -1(220), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.50
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.133
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -0.90  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 214 (3) KHKK
     Found: pos: 215 (3) HKKK
     Found: pos: 216 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
     RNP motif found:  pos.  77 (6) RGVGFVTP
nuc modified.   Score:  0.70
Nuclear Signal   Status: notclr ( 0.40)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
                          nucleus --- Certainty= 0.460(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.198(Affirmative) < succ>


----- The End -----