Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LANSWPFRTI HTYRCQDRKE SLCAPTSKAP QNVSEWPSKQ PSPPSRQRFL
 LRRRTLSLRK TSPYRQTKLR PPLCGRLKKP SGSTWGSLMS ARRPSSAPVM
 TNRKTRSSSS CKITETYSHG NLRICRESQE NWPSTNRSIL RRGRSGKSYV
 VSRPTRERPF EPSLAWSRLD LLEKYYTPSG PTLFLYSKRI KWIGACASTK
 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          200 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   0.36
     Net Charge of CR: 2
     Discriminant Score:    -13.72
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -7.96
     Possible cleavage site: 57
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   9.87  threshold: -2.0
     PERIPHERAL  Likelihood =  9.87
     modified ALOM score:  -2.87
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 16
     YRCQDR
Discrimination of mitochondrial target seq.: 
     negative (-0.64)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.36 at (48)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.04
     score from the 1-31 region:  6.96
Chloroplast protein?  Status: negative (-4.65)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 167(200), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.92
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -8.14  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.5% ( 0.33)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.528(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.200(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.200(Affirmative) < succ>


----- The End -----