Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LSCTNNNLIL MRTSARTTNS PACPFPSQQS LSLSLLQVSK KQVVGNFKPK
 SKRTRLHTCC AQRLRTNEHQ LTFASPKSSP SPLHLLRLRV QERALRICEK
 LGRSHYCLRA SRSDATPCRS LLSASPALQL GSEPASRRSQ AAAARLRACL
 PPPPPRRVRT ARGWTWSTTR RPRMTTAPAS APAQAGPRPP GSWEATTRTT
 PRASGPRWAL DPAPCRRAPA RTPRAGTRRC RTGTWSSSAP SALPGLVRAR
 MSLGRSCASC TPPMAACGTP CTAAREPPAM SGRSRSPHYT CCNLWGGRRN
 RRRRRAAPRR SPRSTRWIGC SGCPACAAAS GCTCSVCSSR WSSYPSPKPA
 LLACRRRRRP RAPTGSAPPT RSFVLSSSRR RCSRRLSGFA SRTLSGSTRM
 PPPPPPRSCR PWSCAHGSFR HCLPWTTGRC PRTYRSSSSE APLLPSPLRN
 RTTTRMPAAE TTSVGTVATS LEASAESARW AGAGGVRTRA CGARGRKGAT
 RRRGGGRKAP GRARRARARR RACRRSPRTC GWAAAPTPSH PSSPRRPGRL
 GGGQRPRRRA GGSGPPGSRG CRRRRRRWRS GPTRRARGRG RGRRRWGGAS
 PRPGASVQEP TPGLRRPPRR AARGGSAGAC AARGHRSRRA RRRGCRRNGR
 WPRPRTPPRT RRPSGSRRMA PSPAPRRPPA RAAGQGPAPR RGSTGHPPAS
 SLARCTPAAA PWPSPSEALA VTWLFAAAEA PAWLDMGGGS IAVAEAAVED
 RGHRPGGTPE LRHREGYPWL PGTHPFHSLP H

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          781 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   10
     Peak Value of UR:   1.23
     Net Charge of CR: 0
     Discriminant Score:     -6.59
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.14
     Possible cleavage site: 20
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.85  threshold: -2.0
     PERIPHERAL  Likelihood =  1.85
     modified ALOM score:  -1.27
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 91
     LRVQER
Discrimination of mitochondrial target seq.: 
     positive ( 3.71)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.59 at (28)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  4.89
     score from the 1-31 region: 11.90
Chloroplast protein?  Status: positive ( 4.76)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 91)  from: 8  to: 11  Score:  3.5
Mitochondrial matrix?  Score:  0.61
Checking apolar signal for intrachloroplastic sorting
     found at (43-45):  2.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-22  Score: 250.88
Chloroplast stroma?  Score: 0.75
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 144(781), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -2.68
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -9.33  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.7% ( 0.34)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 169 (4) RRPR
     Found: pos: 300 (5) RRRR
     Found: pos: 301 (5) RRRR
     Found: pos: 354 (5) RRRR
     Found: pos: 355 (5) RRRR
     Found: pos: 356 (4) RRRP
     Found: pos: 357 (4) RRPR
     Found: pos: 554 (4) RPRR
     Found: pos: 555 (4) PRRR
     Found: pos: 571 (5) RRRR
     Found: pos: 572 (5) RRRR
     Found: pos: 573 (5) RRRR
Checking the 7 residue pattern for Nuclear Targeting
     Found:  pos: 152 (3) PPPRRVR
     Found:  pos: 153 (4) PPRRVRT
     Found:  pos: 154 (5) PRRVRTA
     Found:  pos: 657 (5) PRTRRPS
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  39 (3) KK QVVGNFKPKS KRTRL
     Found:  pos: 215 (3) RR APARTPRAGT RRCRT
     Found:  pos: 500 (3) RR RGGGRKAPGR ARRAR
     Found:  pos: 501 (3) RR GGGRKAPGRA RRARA
     Found:  pos: 506 (3) RK APGRARRARA RRRAC
     Found:  pos: 544 (3) RR PGRLGGGQRP RRRAG
     Found:  pos: 556 (3) RR RAGGSGPPGS RGCRR
     Found:  pos: 557 (3) RR AGGSGPPGSR GCRRR
     Found:  pos: 571 (3) RR RRRRWRSGPT RRARG
     Found:  pos: 572 (3) RR RRRWRSGPTR RARGR
     Found:  pos: 574 (3) RR RWRSGPTRRA RGRGR
     Found:  pos: 645 (3) RR NGRWPRPRTP PRTRR
Final Robbins Score (nucleus):  0.48
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.966(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.894(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.793(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.618(Affirmative) < succ>


----- The End -----