Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KSFEGNFNSS NHLQIFFKLR ILSLATLVNM TRNNLGISFE YSPSDSHCFL
 PKTKQGFVLG NVICATKIKF CRITSFDLGR NQHSSRSCSE GSPRSITKHY
 PCFGVFICFL LLSPWRPVSN EVCRLGLNRR SMNIFNTKFI ELCSPFSNPS
 SSFSVPHNIL QRLRRNDYVV SLKIMPKLSG CHQNSIHLLF CVVFLYAENF
 RYKIYWDLFP GLAIQALLDK CC

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 102 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 109   Charge diffirence(C-N):  0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   1.73
     Net Charge of CR: 0
     Discriminant Score:     -4.01
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.73
     Possible cleavage site: 33
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.53  threshold: -2.0
     PERIPHERAL  Likelihood = -0.53
     modified ALOM score:  -0.79
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 34
     TRNNLG
Discrimination of mitochondrial target seq.: 
     negative (-1.72)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.17 at (27)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  9.16
Chloroplast protein?  Status: negative (-2.43)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(222), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.77
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.303
Amino acid composition tendency for vacuolar proteins
     Score:  1.92  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.303(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----