Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KRRKKNAERH IAKRIAYFNV VNVVQELCGQ PSSGHLDGQE VSVHPNLPSI
 KKMTSGWICK GVYEPVKSKG RTGLILENPN LTEKFFFSSF LSGLKEEVKY
 MVTAQHPTLI VTRLMNMLCT MSHLSQPVRN LEWYLGAEFR KRERGSLQTT
 AFFLLMRFAL ERQKWKETER RLVKPISPKK RKMTLTSTVG KSSFPEWIQS
 NAEVEQYQGS VLGQTESWIR GSFLA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          225 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   1.41
     Net Charge of CR: 6
     Discriminant Score:     -3.71
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.51
     Possible cleavage site: 29
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.72  threshold: -2.0
     PERIPHERAL  Likelihood =  4.72
     modified ALOM score:  -1.84
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 16
     KRIAYF
Discrimination of mitochondrial target seq.: 
     notclr ( 0.80)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.87 at (40)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -1.85
     score from the 1-31 region:  4.87
Chloroplast protein?  Status: negative (-8.65)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 16)  from: 15  to: 18  Score:  2.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 122(225), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   2.37
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.383
Amino acid composition tendency for vacuolar proteins
     Score: -6.49  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:   1 (5) RRKK
     Found: pos: 177 (4) PKKR
     Found: pos: 178 (5) KKRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.700(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.568(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.475(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.180(Affirmative) < succ>


----- The End -----