Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KNTASTACII EPAEDGEIAK EAEEEQAAAG TNGGVIQAKE TVRADDATSP
 VKSIKASTVM QQENGRSASC ISTPVRTGAS DMAAVAMVDK DVSGVTTMSV
 NLMVKNLNVA LHEKRLVPPL PISCVMCRAT ISILDALNDS TFSKSPLIWS
 LFKKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          158 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.55
     Net Charge of CR: 0
     Discriminant Score:    -21.44
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.42
     Possible cleavage site: 38
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.59  threshold: -2.0
     PERIPHERAL  Likelihood =  1.59
     modified ALOM score:  -1.22
(10) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 10
     Uncleavable? Ipos set to: 20
Discrimination of mitochondrial target seq.: 
     negative (-3.77)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.88 at (41)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  6.85
     score from the 1-31 region:  8.87
Chloroplast protein?  Status: positive ( 3.49)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (82-88):  6.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-83  Score: 38010.91
Chloroplast stroma?  Score: 0.75
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(158), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -8.44
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  0.02  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 152 (5) KKKK
     Found: pos: 153 (5) KKKK
     Found: pos: 154 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

               chloroplast stroma --- Certainty= 0.862(Affirmative) < succ>
                          nucleus --- Certainty= 0.700(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.504(Affirmative) < succ>
      chloroplast thylakoid space --- Certainty= 0.449(Affirmative) < succ>


----- The End -----