Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KMKGQRRPTT SKRININSSL QRSSIASGIK IHSSRSLSLL YSSLPHLYLP
 PYLIHNKWLD IDIHIKNFKQ NFGFQNQMRN NSRPNLLYPF SYKSRGRDFC
 GGNLHLKSFW GQKKSNSISQ KFENNISNEL VIIMEILNKH LNCLYINDVL
 LEIICVRYSY LKTFASINKV NNTNKYIYTY LHLCSFRWCI LPCILPVNVT
 TSEIKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          209 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 183 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 190   Charge diffirence(C-N):  1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   0.09
     Net Charge of CR: 4
     Discriminant Score:    -13.70
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.74
     Possible cleavage site: 49
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   0.37  threshold: -2.0
     PERIPHERAL  Likelihood =  0.37
     modified ALOM score:  -0.97
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 37
     SRSLSL
Discrimination of mitochondrial target seq.: 
     notclr ( 0.64)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 14.23 at (58)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.18
     score from the 1-31 region: 11.56
Chloroplast protein?  Status: notclr ( 1.01)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 37)  from: 25  to: 29  Score:  3.0
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
     found at (72-74):  2.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-79  Score: 542.72
Chloroplast stroma?  Score: 0.4
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(209), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.47
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  1.95  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 204 (5) KKKK
     Found: pos: 205 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.600(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.521(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.465(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.281(Affirmative) < succ>


----- The End -----