Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KHFLWPLFSS DHCMCEPFLK IYVYLLIVLL PAKLVFLCHL FHQRSGVGNY
 NRQVFPFLHC ISKKHMTNCF WLDSAFPREI FLFQFSALPH CQIFRNLTML
 KSQVETREIF FASDLIVHAT FYKNFFIVVL CASQLLTIFQ ELDHVVKELK
 KIAIVHLLHV TPTLFARNNT NRETFSLYVS LIFMHHHMTP CLKTINKNIY
 YQIMHHAGFY SVCILQENIK DQKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          226 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 21 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 28   Charge diffirence(C-N):  3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:   2.80
     Net Charge of CR: 1
     Discriminant Score:      2.04
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.96
     Possible cleavage site: 15
... positive value of mtop ...
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -7.43  threshold: -2.0
     INTEGRAL    Likelihood = -7.43   Transmembrane   21 -  37 (  13 -  42)
     PERIPHERAL  Likelihood = -1.91
     modified ALOM score:   1.59
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 38 to 226 (190 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
(10) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 10
     Uncleavable? Ipos set to: 20
Discrimination of mitochondrial target seq.: 
     negative (-3.18)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  5.68 at (37)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  2.11
Chloroplast protein?  Status: negative (-11.02)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

> Relative position of the end of the tail: 16%
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 32(226), count: 1   AKL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   7.18
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score: -0.17  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 222 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.03)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.820(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.388(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.190(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----