Query Information

ORIGIN plant
BEGIN
>MYSEQ
 KEKKKRETHC KKNRLFQCCK LVSGTGVMWS VTLILIGTPL VRSRIGFCAS
 KSVTQLMNEN DIRLDLGSIA SLKKRPDWTD LGKPSYEVFL FLFSKWFKGG
 SEVYGHCSTP YPNSVNKAYE HAMHYELALE SASKKSRVVP RCRIPKERAR
 LTSNYRFLST YEICTRKTEV ERDREETSKA NFTKAENDTN FYCGQVIISR
 MDSVVECSRA VPRFGFGTDR KLDKRIVLS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 21 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 28   Charge diffirence(C-N): -1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:   1.52
     Net Charge of CR: 5
     Discriminant Score:     -4.20
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.77
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.54  threshold: -2.0
     PERIPHERAL  Likelihood = -1.54
     modified ALOM score:  -0.59
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-2.54)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.10 at (56)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.01
     score from the 1-31 region:  3.09
Chloroplast protein?  Status: negative (-5.50)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 149(229), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.63
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -1.50  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:   2 (5) KKKR
     Found: pos:  72 (4) KKRP
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 133 (3) KK SRVVPRCRIP KERAR
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.960(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----