Query Information

ORIGIN plant
BEGIN
>MYSEQ
 IVKLYQSYFN VKNERMTNHE LIVPCMPFPL PAVLAKPKLA IAPSFKETSS
 RKLQTKIQKN TTPHLLCAAS TNQRTSAHFC FSKVKSQSTS LASPARAGAS
 ATDLKAWPKS LLPPRFSKRR HAMPIVAELG VARLAAARIG ASSSPSRRSS
 PASLMSPSSS SSSCSDGAWV DVVVDDAPSA DDDGAGISSC PSRSTLAARL
 LGGDDADDAA GERPTMGTGS VSSLPSRAGE NATGRYSSLS HGNLEFLKCS
 FSASRPCTRA YVTWQILREL YTSHGCLWNA LYSRTRASGD VRLTKPPTLH
 LLLKSMGRTK SAEKGCSSPL ATIHSLDWMQ WLSSMLSSSF WLYLFGMFLI
 MTVVLVSISE AGVACVSAPP PASGSLNRKR ASHPLFRPFQ LPPPLLPTPF
 WVCFPDAVGV DEDAAAAATT ILPPLVLRSW LVPALPPLDD WSLPALDLQE
 LLLRSTTPSL ALTEPDDDED AGGDDVGWDG RHVASLGGVG AVGRCWWGAY
 PRLRGPGPEG RVNSTTRRRP KSSWPLSSAS ARAASRVSKK STNLWLGRCA
 DSLTSFKSPK AWKTWRRSAT ETPGRGLRTT REPRLSPSSA PVAKWAHTAC
 PRTRPRPSSL GWGQSQAWGV GAGAHAGATA AAASCPRRLC WRMCCSRTQV
 KASSAARMSK KWTVATPSDA TAHASTERIA PNGAIACATS ASCACGGTRP
 STTTRLDGPL TTGVVVGKVY PGCGAMAIAI GGTGCNMAIC GGGGTGMAGY
 GWGQYSCCGS GCRGGAQTVG WNSAAASRRL PVASGNPPIP LPPPP

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          795 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 342 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 349   Charge diffirence(C-N): -0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   0.05
     Net Charge of CR: 1
     Discriminant Score:    -14.75
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.86
     Possible cleavage site: 35
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -8.55  threshold: -2.0
     INTEGRAL    Likelihood = -8.55   Transmembrane  342 - 358 ( 338 - 369)
     PERIPHERAL  Likelihood = -0.37
     modified ALOM score:   1.81
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 341 (341 Residues)
(13) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 13
     Uncleavable? Ipos set to: 23
Discrimination of mitochondrial target seq.: 
     negative (-3.20)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.26 at (25)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  3.45
Chloroplast protein?  Status: negative (-8.95)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 42%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.362
SKL motif (signal for peroxisomal protein): 
     pos: 198(795), count: 2   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   0.53
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.137
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -2.51  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 117 (3) KRRH
     Found: pos: 516 (4) RRRP
     Found: pos: 517 (4) RRPK
Checking the 7 residue pattern for Nuclear Targeting
     Found:  pos: 113 (3) PRFSKRR
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.65
Nuclear Signal   Status: notclr ( 0.35)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.396(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.362(Affirmative) < succ>


----- The End -----