Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ISGNSGTETC YPVPNIILGF ETDPNCLVNS DSGRSGTGSG SSGLDNGSDN
 GYLAHPLEYR SAHLNMEPLT CSSMDWKLQA PQTSTILTRN CTIRLDRSKC
 LGNFGSTGIT DSFGSTSRQI LIPCSSQPMH FFTSHQLYPV QSPELIAGHR
 LTSRRSGNRQ SGTQALQEFW HHHLFHGHKR RSNPPPDVQA RRVMKGQQCS
 AYTWKFTQNG IAITYTGH

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          218 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   12
     Peak Value of UR:   1.79
     Net Charge of CR: -1
     Discriminant Score:     -2.72
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -7.16
     Possible cleavage site: 39
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   8.75  threshold: -2.0
     PERIPHERAL  Likelihood =  8.75
     modified ALOM score:  -2.65
(20) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 20
     Uncleavable? Ipos set to: 30
Discrimination of mitochondrial target seq.: 
     negative (-4.34)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.88 at (67)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  4.95
     score from the 1-31 region:  6.41
Chloroplast protein?  Status: notclr (-0.30)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (51-54):  2.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-57  Score: 890.88
Chloroplast stroma?  Score: 0.4
SKL motif (signal for peroxisomal protein): 
     pos: 62(218), count: 1   AHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   1.52
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.269
Amino acid composition tendency for vacuolar proteins
     Score: -6.03  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 177 (3) HKRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 178 (3) KR RSNPPPDVQA RRVMK
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.880(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.488(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.400(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>


----- The End -----