Query Information

ORIGIN plant
BEGIN
>MYSEQ
 IPNGCTKVTT AHRSEPTAIH RNYSPTATVR VRSSETIPKA CASPGALPPQ
 LSQDFFELAF RVAAQILVPA ACCLDTHALC IYFLLLLCHL LAFLADAPEF
 LLELSCGSIF FIEQTDSGGG SVLRLTQLAD LSMDTGALRF DLDQFGQDAL
 YRLHTESICL QKGDCPDLVL EPFHAVVY

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          178 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 58 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 65   Charge diffirence(C-N):  1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:  -0.93
     Net Charge of CR: 1
     Discriminant Score:    -22.78
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.83
     Possible cleavage site: 48
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -9.39  threshold: -2.0
     INTEGRAL    Likelihood = -9.39   Transmembrane   78 -  94 (  70 - 102)
     INTEGRAL    Likelihood = -2.44   Transmembrane   58 -  74 (  58 -  74)
     PERIPHERAL  Likelihood =  4.19
     modified ALOM score:   1.98
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
 count: 1)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 24
     (count:1)
     HRNYSP
Discrimination of mitochondrial target seq.: 
     negative (-2.76)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.69 at (25)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  5.96
     score from the 1-31 region:  6.35
Chloroplast protein?  Status: notclr ( 0.03)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (78-95): 11.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-95  Score: 21954.56
Chloroplast thylakoid memb.?  Score: 0.594
SKL motif (signal for peroxisomal protein): 
     pos: -1(178), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.17
Peroxisomal proteins?   Status: negative
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -0.80  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

   chloroplast thylakoid membrane --- Certainty= 0.636(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----