Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ILGGSNIGRS ISSGGLSMPG IASRMNLSGN SGSGAINIQG SNRIGMLQQA
 SPQFMNLLGS SYPTPGGSLS QNQVQSGSGS LGSSGMLYEG SSGDNAPFVL
 IQASGILFIR TTLFSITKQW RRDLSFLLLA KILGENYKVL LHLKLIWFDQ
 LLSWMGVHTK FYKNLPPGGS CRNVAEIIFF IICWHYLVGP LLYQCCDLIC
 CCCCCDAVTV MIINVLTVMS QHEKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          228 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 98 at i=3
MTOP: membrane topology (Hartmann et al.)
     I(middle): 105   Charge diffirence(C-N):  5.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   14
     Peak Value of UR:   1.10
     Net Charge of CR: 1
     Discriminant Score:     -5.58
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.8
     Possible cleavage site: 22
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -5.41  threshold: -2.0
     INTEGRAL    Likelihood = -5.41   Transmembrane  199 - 215 ( 194 - 220)
     INTEGRAL    Likelihood = -3.24   Transmembrane  177 - 193 ( 173 - 193)
     PERIPHERAL  Likelihood = -0.96
     modified ALOM score:   1.18
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 45
     NRIGML
Discrimination of mitochondrial target seq.: 
     negative (-3.63)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.62 at (30)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region: 12.58
Chloroplast protein?  Status: notclr ( 0.31)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (44-47):  3.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-19  Score: 1187.84
Chloroplast thylakoid memb.?  Score: 0.355
SKL motif (signal for peroxisomal protein): 
     pos: -1(228), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.06
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -0.15  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 223 (5) KKKK
     Found: pos: 224 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.06)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.439(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----