Query Information

ORIGIN plant
BEGIN
>MYSEQ
 IFWVGAILGE VALEDCLCLV LHLAIVATLV VGLSTFKDPT VLVCFNKHPH
 NLTCLEVLTQ HLEGHYLRIK YSQEVVLDLL ECYMKEAQVT MLHLFSKHLG
 YCSGQLCSPL RSSGEETCPS FCWPKYARTI RFSCISFGLI SSCCHGWAEF
 IQNDSIRTYD PQVAVVEMLR FRFSSFVGII GHCCTNVVTF VVVVVVTQSP
 MFPLVSMKKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          212 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 17 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 24   Charge diffirence(C-N):  4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   2.86
     Net Charge of CR: -1
     Discriminant Score:      2.05
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.58
     Possible cleavage site: 29
... positive value of mtop ...
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value: -10.08  threshold: -2.0
     INTEGRAL    Likelihood =-10.08   Transmembrane   17 -  33 (  10 -  37)
     INTEGRAL    Likelihood = -5.73   Transmembrane  179 - 195 ( 176 - 200)
     PERIPHERAL  Likelihood =  3.87
     modified ALOM score:   2.12
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
( 9) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 9
     Uncleavable? Ipos set to: 19
Discrimination of mitochondrial target seq.: 
     negative (-0.79)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.56 at (61)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  2.39
Chloroplast protein?  Status: negative (-8.70)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(212), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.86
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -2.18  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 207 (5) KKKK
     Found: pos: 208 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.01)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----