Query Information

ORIGIN plant
BEGIN
>MYSEQ
 HQTSHIYITD IITNINIQHK HKYSSSQRKH KYQEHKSHKS QALTVSNTNI
 SIQHKFSLNT ENITLMEVGG STGATLGTLH EGCWMPPQIG PAQRRDCMCY
 TRCITNILQF YSQEYGRQGQ LQGTMEVAES PVQHQGSACT GTSPPSSYDL
 PGEPPAPPSS SPPSHDVPSS LLLVGYVILQ ANVIVTQIEG ILKEGR

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          196 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 170 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 177   Charge diffirence(C-N):  1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   0.85
     Net Charge of CR: 1
     Discriminant Score:     -9.15
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -8.98
     Possible cleavage site: 48
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.03  threshold: -2.0
     INTEGRAL    Likelihood = -3.03   Transmembrane  170 - 186 ( 169 - 188)
     PERIPHERAL  Likelihood =  6.26
     modified ALOM score:   0.71
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 187 to 196 (11 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 30
     QRKHKY
Discrimination of mitochondrial target seq.: 
     negative (-3.79)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.10 at (42)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.92
     score from the 1-31 region:  8.29
Chloroplast protein?  Status: notclr ( 0.90)

*** Reasoning Step: 2

> Relative position of the end of the tail: 95%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (68-79):  4.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-94  Score: 2908.16
Chloroplast thylakoid memb.?  Score: 0.212
SKL motif (signal for peroxisomal protein): 
     pos: -1(196), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -3.21
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -7.26  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  27 (3) RKHK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.15)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.361(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.190(Affirmative) < succ>


----- The End -----