Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GWGREWNGWV PGSHGPSRCR SSGVPPYGLC PLSSTAASAT AILPPPISSH
 AGASAAANSH VTASASDGDG HGAAAGVHLA NDDAGGQWPV EPRRGAGPCP
 AARAGGRRGA GDGAIRRDPL GRRVRGGVRG RGHRPFLRHP RRRARLDLCP
 RAAHAPAEPP RAALRGGRRS PGVGSCTDAP GLGLAPPQRR RPRPRPRARR
 VGPLRHRRRR RRQPRLPGGP EPPARRLGRP PPSLPGLRGL EGCEGVGAAA
 QPQVRGLLRH ARRRARARRA QRPGAFRPPP RRRVHAPFRP RAPQARVRTP
 PAPAHRADSA EASSDVATVP TDVVSAAGIL VVVRFRKGEG RSGASEEELL
 VRGQRPVVQG RQCRNEPAQD QGRQDRGGGG GGILVDPDSV REANPERRRE
 QRRRELERTK ERVGGALPVQ GARGRRRRRH ASNAGFGDGY EDHRHDQEHT
 EQVQPEAAAQ HAGQPLHPIQ RVDRGERRGA ALLRLRFRLP PHRFQQQVCG
 LRLRQPDIAG GSRAAVQGVP QAAMGGVQLA QDLPSDIRAR TRPGSAEGAL
 QELQVPVRQR RVPARGVLAG ARRQGAYGSS AHRGPLARGV VRVVASQEPG
 GRGPAWAGAD AGAVVIRGRR VVDHYVHPRA VRTRRGGGGG RHQARRRAAA
 AWLRRLAGSD PSCSAGDALS NDRHGVASLR EARRQLRPSF SQIRSARSCT
 RRRSKSGLGL DFGEAKVSCS LVRRRCAQQV WSRVLLDFGL KFPTTCFFET
 WSYSLRLSDC WEGKGHAGDY QFVVRHALVL YIKIRLLLVQ LYN

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          793 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 316 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 323   Charge diffirence(C-N):  4.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:  -0.44
     Net Charge of CR: 0
     Discriminant Score:    -18.82
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.13
     Possible cleavage site: 41
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.28  threshold: -2.0
     INTEGRAL    Likelihood = -2.28   Transmembrane  316 - 332 ( 316 - 333)
     PERIPHERAL  Likelihood = -1.22
     modified ALOM score:   0.56
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 333 to 793 (462 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 22
     CRSSGV
Discrimination of mitochondrial target seq.: 
     negative (-4.84)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.57 at (29)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  3.90
Chloroplast protein?  Status: negative (-9.62)

*** Reasoning Step: 2

> Relative position of the end of the tail: 41%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.111
SKL motif (signal for peroxisomal protein): 
     pos: 144(793), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -1.32
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -10.82  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 139 (4) PRRR
     Found: pos: 188 (4) RRRP
     Found: pos: 189 (4) RRPR
     Found: pos: 204 (3) RHRR
     Found: pos: 205 (3) HRRR
     Found: pos: 206 (5) RRRR
     Found: pos: 207 (5) RRRR
     Found: pos: 208 (5) RRRR
     Found: pos: 279 (4) PRRR
     Found: pos: 424 (5) RRRR
     Found: pos: 425 (5) RRRR
     Found: pos: 426 (3) RRRH
Checking the 7 residue pattern for Nuclear Targeting
     Found:  pos: 186 (5) PQRRRPR
     Found:  pos: 278 (5) PPRRRVH
     Found:  pos: 279 (5) PRRRVHA
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 198 (3) RR VGPLRHRRRR RRQPR
     Found:  pos: 267 (3) RR AQRPGAFRPP PRRRV
     Found:  pos: 618 (3) RR VVDHYVHPRA VRTRR
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.55)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                          nucleus --- Certainty= 0.910(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>


----- The End -----