Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GTSSCAAAPI PSTLAATPLP SPPLQVMSFL AGSSSCGCKF RLENWWEEGR
 RKGGTQGGGL PDRTGFAQTS GTYMLGMRGV AARPLSLSYG CPSASMILET
 CQRMFVFFTS HAHWLIQFMS YVLSFASRLI ILSMFFVMIH VYTLLPPPLC
 YFAKEMLSKF YIWMSEVF

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          168 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 129 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 136   Charge diffirence(C-N):  0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   37
     Peak Value of UR:   1.15
     Net Charge of CR: 0
     Discriminant Score:      1.12
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.9
     Possible cleavage site: 36
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 37
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -7.22  threshold: -2.0
     INTEGRAL    Likelihood = -7.22   Transmembrane  129 - 145 ( 120 - 147)
     PERIPHERAL  Likelihood =  5.67
     modified ALOM score:   1.54
>>> Seems to be a Type Ia membrane protein
     The cytoplasmic tail is from 146 to 168 (23 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 43
     FRLENW
Discrimination of mitochondrial target seq.: 
     negative (-1.08)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.67 at (34)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.88
     score from the 1-31 region: 11.82
Chloroplast protein?  Status: positive ( 3.69)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
     found at (79-82):  3.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-8  Score: 10813.44
SKL motif (signal for peroxisomal protein): 
     pos: 127(168), count: 1   SRL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  12.72
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score: -2.09  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Type Ia is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.460(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.388(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.280(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----