Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GTSAATPAPA SPRTAPSSKT TATSTRASSA SAPTRTTSPS ATSPSRYTRS
 MPLAPYHRNC RILRASIWIA RITQVLCHLS SGILLCSTLW APMHCLDLFQ
 RSLGTLILYL ALARTTMAPF LGSWETWQNW SNSMRHLWPT LGKIRPLLMA
 MLAASSSEEE SVELSSESHS RPICVSPPFL YFFFSIFFFP FLSSSLSLSL
 VYGHLAIKSD LPQKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          218 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 75 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 82   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   0.31
     Net Charge of CR: 0
     Discriminant Score:    -12.66
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.51
     Possible cleavage site: 36
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.24  threshold: -2.0
     INTEGRAL    Likelihood = -3.24   Transmembrane  173 - 189 ( 172 - 191)
     PERIPHERAL  Likelihood = -0.00
     modified ALOM score:   0.75
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 172 (172 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 147
     IRPLLM
Discrimination of mitochondrial target seq.: 
     negative (-1.07)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.49 at (58)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.01
     score from the 1-31 region: 16.69
Chloroplast protein?  Status: positive ( 5.65)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 78%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (79-86):  4.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-10  Score: 7536.64
Chloroplast thylakoid memb.?  Score: 0.411
SKL motif (signal for peroxisomal protein): 
     pos: -1(218), count: 0   
Amino Acid Composition Tendency for Peroxisome:   3.79
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.572
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -1.29  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 213 (5) KKKK
     Found: pos: 214 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.803(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.665(Affirmative) < succ>
      chloroplast thylakoid space --- Certainty= 0.665(Affirmative) < succ>


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