Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GTRYNLASYV ARGGVRCGFI PLEVRQRTYA LHSYAFIEVI FADDVVLYSQ
 RYEDRVPTHC IPMHTLKYLQ VLLTRGGVIE PIVVVRGNEM ILVILGLLSS
 ISTSISDLDC DSFKMLINSI CGLNISSEQL ACDVSSHSCI TQCRCLLLIK
 GSGMQHIHLH SHNNAAQTQS FNKLLVKGCL FFNSCTLINS VHVVMVVFPL
 LVDYCLLCFL IMISIYTLLH PGLCNFQRRC RNLKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          237 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 82 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 89   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   1.04
     Net Charge of CR: 1
     Discriminant Score:     -7.84
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.72
     Possible cleavage site: 42
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.28  threshold: -2.0
     INTEGRAL    Likelihood = -8.28   Transmembrane  197 - 213 ( 190 - 224)
     INTEGRAL    Likelihood = -4.35   Transmembrane   82 -  98 (  81 -  99)
     PERIPHERAL  Likelihood =  0.26
     modified ALOM score:   1.76
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
 count: 1)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 30
     (count:1)
     QRTYAL
Discrimination of mitochondrial target seq.: 
     notclr (-0.27)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.08 at (67)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  4.85
Chloroplast protein?  Status: negative (-8.80)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 30)  from: 39  to: 42  Score:  4.0
Mitochondrial inner membrane?  Score:  0.70
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.351
SKL motif (signal for peroxisomal protein): 
     pos: -1(237), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -3.54
Peroxisomal proteins?   Status: negative
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -0.58  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 233 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.03)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.702(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.351(Affirmative) < succ>


----- The End -----