Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GRTNPSGQTD VIVFCSGGFE KLGTTESEGE LLSQLVDLLK LSGARHTILY
 ASQPSGYLLE NPSGSPVARH LAERTNGTKT GLLSKCDGEC LVKSTLLEGT
 FVGIVLLIIL ISGLMCMMGI DTPSKFEAPQ ESSPFEAEPL FCWSALWIDI
 IQFPPLKAPF VLLLFFYAIC YLRCAQTFDT KRMHVCEACA VTRHSIASRC
 FLITWNFRWV LCIKKRNLAV QKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          226 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 101 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 108   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   9
     Peak Value of UR:   2.08
     Net Charge of CR: 0
     Discriminant Score:     -0.95
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.5
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value: -11.78  threshold: -2.0
     INTEGRAL    Likelihood =-11.78   Transmembrane  101 - 117 (  90 - 123)
     INTEGRAL    Likelihood = -3.82   Transmembrane  156 - 172 ( 153 - 172)
     PERIPHERAL  Likelihood =  4.67
     modified ALOM score:   2.46
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     GRTNPS
Discrimination of mitochondrial target seq.: 
     negative (-4.26)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.31 at (31)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.02
     score from the 1-31 region:  5.38
Chloroplast protein?  Status: negative (-5.26)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.491
SKL motif (signal for peroxisomal protein): 
     pos: -1(226), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.77
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -0.77  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 221 (5) KKKK
     Found: pos: 222 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.06)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.491(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----