Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GRDKLPFLSD QKSYRYPRQS KSSMGITLSR SRICLYTNLR QLKRAIPTMV
 LLNPLRLFIS AMVEALVPCR CQIEIKQTCF RGDQIYQIWY SNLLRSIGEE
 GHITLTDPKR KTSINLFPRA SPAGILFSAA LQIRATLEKR RPGSWYENRT
 ISGVRSRSKQ SGLIERSISK RRDRQALRPV QKQKERSWGL RLLILWLIAI
 EVVLRIFLWR LA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          212 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 50 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 57   Charge diffirence(C-N): -3.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:   0.45
     Net Charge of CR: 0
     Discriminant Score:    -14.75
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.41
     Possible cleavage site: 61
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -9.55  threshold: -2.0
     INTEGRAL    Likelihood = -9.55   Transmembrane  192 - 208 ( 187 - 211)
     PERIPHERAL  Likelihood =  0.32
     modified ALOM score:   2.01
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 191 (191 Residues)
( 2) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 2
     Uncleavable? Ipos set to: 12
Discrimination of mitochondrial target seq.: 
     negative (-3.44)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.91 at (65)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  9.75
Chloroplast protein?  Status: negative (-1.35)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 90%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.402
SKL motif (signal for peroxisomal protein): 
     pos: -1(212), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.76
Peroxisomal proteins?   Status: negative
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -9.22  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 107 (4) PKRK
     Found: pos: 138 (4) KRRP
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 169 (3) KR RDRQALRPVQ KQKER
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.50)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
                          nucleus --- Certainty= 0.800(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.402(Affirmative) < succ>


----- The End -----