Query Information

ORIGIN plant
BEGIN
>MYSEQ
 GLALCGDKSS SCFSWSDYLF TNRKPGFSIN PSYPDVPFPN GKNTTYQHHS
 HNQNHRSHSH LYHNVSDQVP LIARAPLAAH NREHGYISFQ TLCRGCALYP
 QAVIPILPGD HDSPLITTKV TQQGITTPLQ RFPGAVATRV SMHRTPPQGA
 NPNLAERASY TEPHRHDDVN YTLDPLIIQL RASHSTLMVE LFSRAIIHRT
 GPYGEA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          206 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   0.26
     Net Charge of CR: 0
     Discriminant Score:    -13.93
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.68
     Possible cleavage site: 14
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   3.87  threshold: -2.0
     PERIPHERAL  Likelihood =  3.87
     modified ALOM score:  -1.67
( 6) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 6
     Uncleavable? Ipos set to: 16
Discrimination of mitochondrial target seq.: 
     negative (-1.00)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.80 at (26)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region:  9.79
Chloroplast protein?  Status: notclr ( 0.74)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (69-73):  3.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-78  Score: 12451.84
Chloroplast stroma?  Score: 0.4
SKL motif (signal for peroxisomal protein): 
     pos: 59(206), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   0.22
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.096
Amino acid composition tendency for vacuolar proteins
     Score: -3.28  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
>>> Seems to be N-myristoylated  (0.10)
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

               chloroplast stroma --- Certainty= 0.505(Affirmative) < succ>
                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.367(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.174(Affirmative) < succ>


----- The End -----