Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FLVDAHAPIH FILFEYKNRV GPLGVYFSNK SSRDQASLGS NGLSLVGRET
 TLLPDRPRLR IDLQFVLGQF SWDSRHIRRL PCEYVSVILQ ELDERVFLFV
 IQTGADDGRL ALISEPQVDP LGFFSRPHRG HGLSFVCRYG EVFLRLRVRR
 RRRNRRLGGE GCLDGHSETF CGALEVGAHS YDSLRSWHLQ YHVCIVRNGH
 ELAS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          204 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   1.41
     Net Charge of CR: 1
     Discriminant Score:     -6.44
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.24
     Possible cleavage site: 54
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.80  threshold: -2.0
     PERIPHERAL  Likelihood =  1.80
     modified ALOM score:  -1.26
( 3) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 3
     Uncleavable? Ipos set to: 13
Discrimination of mitochondrial target seq.: 
     negative (-2.22)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.61 at (54)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  4.08
Chloroplast protein?  Status: negative (-6.43)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(204), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.48
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -4.20  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 148 (5) RRRR
     Found: pos: 149 (5) RRRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                          nucleus --- Certainty= 0.600(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----