Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFSFFFFS SSSVSFCRGL NPFFSLQPKQ RYCQPLKIRL NKSCYRKSRN
 LYDDCGALRT NFEIRSDPLE RRDKLSRLYL NFQIGYDMYP WWQKRTRDVF
 GDGGFRGDQN VVELKTLKET KTSNSTQPMQ PKTQQALTKY MLNGLDCGKT
 NLLFFGFFWT IGDKKTAKKS KSLTDKPCSD YQLMCTRWVI ARSFDERVWN
 NSIGGGDDVH GPTTAFQRRC ALATDTPPPM A

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          231 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   16
     Peak Value of UR:   2.35
     Net Charge of CR: 0
     Discriminant Score:      3.10
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.59
     Possible cleavage site: 19
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 20
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   3.76  threshold: -2.0
     PERIPHERAL  Likelihood =  3.76
     modified ALOM score:  -1.65
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 51
     SRNLYD
Discrimination of mitochondrial target seq.: 
     positive ( 4.56)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.88 at (34)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region: 11.72
Chloroplast protein?  Status: notclr ( 0.05)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Number of Potential N-glycosylation Sites: 3
Out: score 0.300
Checking apolar signal for intramitochondrial sorting
  (Gavel position 51)  from: 1  to: 9  Score:  7.5
Checking apolar signal for intrachloroplastic sorting
     found at (55-58):  3.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-62  Score: 614.40
SKL motif (signal for peroxisomal protein): 
     pos: 76(231), count: 1   SRL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   3.20
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.140
Amino acid composition tendency for vacuolar proteins
     Score: -7.68  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          outside --- Certainty= 0.370(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.226(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.100(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----