Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFMFSFSF FSNSILNSDL NSNLVPVYPQ FILNKKYQSK YNHIYVVYIL
 YPLFLFSIFL KSPLTLKFSS RYCILFILYI YIFFCIVTKY TPQTQYPACI
 ILMAYILLLI ICFIWCSHRM MHKGIHIRRN YFSIGIISSK LQSGCYKSYP
 PKSRPRDLRR SREKVGKIYA KLFFSFPSCF IFTVVTPLHF AHLYHLISCN
 SSQSIQNLDR VFRVSQITLN TEFFHG

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          226 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 4
     Position of the most N-terminal TMS: 44 at i=3
MTOP: membrane topology (Hartmann et al.)
     I(middle): 51   Charge diffirence(C-N): -0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   17
     Peak Value of UR:   2.24
     Net Charge of CR: 0
     Discriminant Score:      2.62
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.45
     Possible cleavage site: 14
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 15
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 4  value: -14.49  threshold:  0.5
     INTEGRAL    Likelihood =-14.49   Transmembrane   98 - 114 (  94 - 122)
     INTEGRAL    Likelihood = -8.60   Transmembrane   72 -  88 (  68 -  91)
     INTEGRAL    Likelihood = -6.53   Transmembrane   44 -  60 (  43 -  62)
     INTEGRAL    Likelihood = -2.07   Transmembrane  172 - 188 ( 172 - 188)
     PERIPHERAL  Likelihood =  4.29
     modified ALOM score:   3.50
>>> Likely a Type IIIa membrane protein (clv)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 156
     SRPRDL
Discrimination of mitochondrial target seq.: 
     positive ( 2.70)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.66 at (62)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region: 11.29
Chloroplast protein?  Status: negative (-0.72)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 156)  from: 44  to: 60  Score:  9.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 191(226), count: 3   AHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   5.85
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  1.35  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.370(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----