Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFLVFNIF LSQNAHRIKS SMMHNLVVYI FINCFAWCSH MMMHEDQTHI
 KRKCFSIGII SSKLQSGCYM KMNDCYFLKL FNYMVKFLKD CQLSTNYNKK
 VLVKSSGMDY QVTCKKNLSC FYLLALQLHC QVPKNLLTMW KCRELEKYFS
 WKCTIKSPKT ICHVLLLANT MKEWKHLPVV SVPYSRSLME QVTQKHLRWQ
 HNQQIYIDLQ KWLTHAVIRS KERPKML

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          227 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 26 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 33   Charge diffirence(C-N): -0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   14
     Peak Value of UR:   2.65
     Net Charge of CR: 0
     Discriminant Score:      4.59
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.08
     Possible cleavage site: 38
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.43  threshold: -2.0
     PERIPHERAL  Likelihood = -0.43
     modified ALOM score:  -0.81
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 19
     HRIKSS
Discrimination of mitochondrial target seq.: 
     positive ( 1.93)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.19 at (48)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  7.19
Chloroplast protein?  Status: negative (-3.72)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 19)  from: 1  to: 7  Score:  7.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 62(227), count: 1   SKL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   3.35
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.257
Amino acid composition tendency for vacuolar proteins
     Score: -3.29  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.550(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.331(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
                          outside --- Certainty= 0.100(Affirmative) < succ>


----- The End -----