Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFLISLQV YVTFTGKIQG KIHQRKDQHK CKYVYIYLFV LFTLLIDYAN
 VFKYEYRTHI IYSKRTSFMY RQFKCLFKIS IIITNSFEML FSNFYWEILF
 DFFCPQNDFR CYKFYPPQKS RPRDLEKGYN KFGLEFFLIW FWKPKFCLKF
 FIWISMSSHL LCQMRYGYGK YRCGKEEYNK ERLHLELWIL IPLAIQLDLR
 LELMFILFDV VGLLWPFIL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          219 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 36 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 43   Charge diffirence(C-N): -4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   15
     Peak Value of UR:   2.81
     Net Charge of CR: 0
     Discriminant Score:      6.03
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.27
     Possible cleavage site: 15
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -7.11  threshold: -2.0
     INTEGRAL    Likelihood = -7.11   Transmembrane  203 - 219 ( 201 - 219)
     INTEGRAL    Likelihood = -2.97   Transmembrane   36 -  52 (  33 -  52)
     PERIPHERAL  Likelihood =  0.63
     modified ALOM score:   1.52
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 27
     QRKDQH
Discrimination of mitochondrial target seq.: 
     notclr (-0.17)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.51 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  3.36
Chloroplast protein?  Status: negative (-7.74)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 27)  from: 34  to: 46  Score:  8.5
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 158(219), count: 1   SHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  16.02
Peroxisomal proteins?   Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -0.25  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----