Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFLCFPFP FSFQIPFIQI IQILCQFILN LYCEIKSTNL RSIITYMLFI
 FYTLYSFSPY FSPLLLNSHL GINVFSLFYI FIYFFVSQNT LHKHNIQHDA
 FWHIFFYLFA FEYGVHIGCI KVKSTGEIIS LLVLFLANYK VGVTNPTPLK
 NNLVLEIEDL GKRWGKSMRS SSSLSQVASF SPWLHCTLHI FITLFLVTRV
 KVSKILIGYS VVKSPTLSSS MGN

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          223 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 5
     Position of the most N-terminal TMS: 15 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 22   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   32
     Peak Value of UR:   2.34
     Net Charge of CR: 0
     Discriminant Score:      7.90
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.97
     Possible cleavage site: 14
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 15
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 5  value:  -4.94  threshold:  0.5
     INTEGRAL    Likelihood = -4.94   Transmembrane   70 -  86 (  69 -  88)
     INTEGRAL    Likelihood = -2.13   Transmembrane   15 -  31 (  15 -  31)
     INTEGRAL    Likelihood = -2.07   Transmembrane  128 - 144 ( 127 - 144)
     INTEGRAL    Likelihood = -0.90   Transmembrane  190 - 206 ( 190 - 207)
     INTEGRAL    Likelihood = -0.00   Transmembrane  104 - 120 ( 104 - 120)
     PERIPHERAL  Likelihood =  1.70
     modified ALOM score:   1.59
>>> Likely a Type IIIa membrane protein (clv)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 43
     LRSIIT
Discrimination of mitochondrial target seq.: 
     notclr (-0.12)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.33 at (28)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region:  3.86
Chloroplast protein?  Status: negative (-5.31)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 43)  from: 70  to: 86  Score:  8.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 68(223), count: 1   SHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   8.87
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  2.08  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.370(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----