Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFLCFLFS HFSFKSQLQF LFVLDFNRIH NKQIKYPVYA SIFFTNYLTS
 LFENLFQICN AHMKCLFLRK YGFKYFSNFI KIFITLFGEV MYFFSIMSYF
 NTKNILYTVL EGDNPFIISN LQTEFLFLFY LNYCFNVSIY FKMVGSKVLF
 IRDTLLFLLL FPKIRFRALQ ILPPKSRPRD LEKGYNRYGL EFFLIGFWKS
 KFCSRFFYIT VSRRFGTYGY CMSSHLLCH

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 82 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 89   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   1.77
     Net Charge of CR: 1
     Discriminant Score:     -4.21
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.510001
     Possible cleavage site: 13
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.75  threshold: -2.0
     PERIPHERAL  Likelihood = -1.75
     modified ALOM score:  -0.55
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 71
     LRKYGF
Discrimination of mitochondrial target seq.: 
     positive ( 1.92)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.66 at (27)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region:  5.85
Chloroplast protein?  Status: negative (-2.95)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 71)  from: 1  to: 9  Score:  8.5
Mitochondrial matrix?  Score:  0.41
Mitochondrial intermemb.space?  Score:  0.30
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 224(229), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  17.18
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  3.84  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
     RNP motif found:  pos.  68 (6) RKYGFKYF
Nuclear Signal   Status: negative ( 0.07)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.580(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.504(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.292(Affirmative) < succ>


----- The End -----