Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFHSFFFL LLLFLFAGAT LFFHYDNPNN KDIANSPLKS DLTNLVNIES
 PEISTMIAER SERILRGQIR WKEDKISFPD CITSKSDMIC IRGGKNEPEM
 FLVMVDFVVT KTWNSKLRNK LRPATRHNRC NQKLNKPLSS TSKCSMVYRI
 AVKLIYYFLA FSGLEIRKQR RKVNLSPINL ALITNCAPVG LPDLSMRGCG
 ITRLGEETTF TARLQPSKDA APQPIHHLLW L

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          231 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 7 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 14   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   18
     Peak Value of UR:   3.43
     Net Charge of CR: 1
     Discriminant Score:     11.90
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 7.12
     Possible cleavage site: 21
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 22
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 1B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.11  threshold: -2.0
     PERIPHERAL  Likelihood = -0.11
     modified ALOM score:  -0.88
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
(25) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     Uncleavable? Ipos set to: 35
Discrimination of mitochondrial target seq.: 
     positive ( 2.78)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.44 at (68)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  4.58
Chloroplast protein?  Status: negative (-7.20)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Number of Potential N-glycosylation Sites: 1
Out: score 0.800
Checking apolar signal for intramitochondrial sorting
  (Gavel position 35)  from: 1  to: 23  Score: 15.5
>>> Seems to have an intramitochondrial signal
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 212(231), count: 2   ARL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  -3.86
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -0.59  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          outside --- Certainty= 0.820(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.100(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----