Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFWSLIF SCHKMHTENP ACIIWYIFLL IVFRHGVHIC MKIKLTRENV
 SLLVLFLANY KVGVTSRTIA IFLSSLTTWS SSKIVNNCEA HRTTIKKFLK
 VQAWTIKSLA KKISRVSTCL FNYIVKFLKI CQCGSAENWN KNISLGNALS
 SHQKQFVMCF YLLIQRNGNT CLLYQFPTPD LWNKHRNTNF AGSNTINRYT
 IFKNGSHMQS DHEKRGHKKC L

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          221 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 21 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 28   Charge diffirence(C-N):  3.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   2.22
     Net Charge of CR: 0
     Discriminant Score:      0.71
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.569999
     Possible cleavage site: 39
... positive value of mtop ...
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.56  threshold: -2.0
     INTEGRAL    Likelihood = -3.56   Transmembrane   21 -  37 (  20 -  40)
     PERIPHERAL  Likelihood =  0.90
     modified ALOM score:   0.81
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 38 to 221 (185 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 200
     NRYTIF
Discrimination of mitochondrial target seq.: 
     negative (-0.83)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.17 at (41)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  2.59
Chloroplast protein?  Status: negative (-7.76)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

> Relative position of the end of the tail: 17%
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(221), count: 0   
Amino Acid Composition Tendency for Peroxisome:   6.01
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score: -1.65  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.820(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.320(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.190(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----