Query Information
ORIGIN plant
BEGIN
>MYSEQ
FFFFFNFTSS LCYILNLGNT GNTPTKRSTM QICVYIFVCI IYFINLRKCF
QIIPHTYYLF QENIILNVTI MFIQNFHNYY FILRDVIFKF LLGDTLVFLL
LTPKLGFVLI LPPLTEISSS RFVRKGIVWS RILVSHLVLE TKVLFKVFYM
DIYVPFIMSD EVRLWQVVWQ GRVGKTSSRT VDLDSAGDST SLKTVDVYPL
CGWSSLAFHL
Result Information
PSORT --- Prediction of Protein Localization Sites
version 6.4(WWW)
MYSEQ 210 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5
*** Reasoning Step: 1
Preliminary Calculation of ALOM (threshold: 0.5)
count: 3
Position of the most N-terminal TMS: 30 at i=2
MTOP: membrane topology (Hartmann et al.)
I(middle): 37 Charge diffirence(C-N): 0.5
McG: Examining signal sequence (McGeoch)
Length of UR: 24
Peak Value of UR: 1.65
Net Charge of CR: 0
Discriminant Score: 0.65
GvH: Examining signal sequence (von Heijne)
Signal Score (-3.5): -5.27
Possible cleavage site: 14
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
count: 2 value: -5.79 threshold: -2.0
INTEGRAL Likelihood = -5.79 Transmembrane 95 - 111 ( 94 - 115)
INTEGRAL Likelihood = -5.68 Transmembrane 30 - 46 ( 28 - 50)
PERIPHERAL Likelihood = 0.42
modified ALOM score: 1.26
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Rule: vesicular pathway
Rule: vesicular pathway
Rule: vesicular pathway
Gavel: Examining the boundary of mitochondrial targeting seq.
motif at: 49
LRKCFQ
Discrimination of mitochondrial target seq.:
notclr ( 1.18)
Hydrophobic moment analysis for chloroplast proteins
Hmax: 9.36 at (62)
Disc.Score from Amino Acid Composition (chloroplast)
score from the 3-11 region: 1.01
score from the 1-31 region: 6.41
Chloroplast protein? Status: negative (-4.67)
Rule: vesicular pathway
Rule: vesicular pathway
*** Reasoning Step: 2
Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL Count: 0
Checking apolar signal for intramitochondrial sorting
(Gavel position 49) from: 32 to: 44 Score: 9.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein):
pos: 135(210), count: 1 SHL
SKL score (peroxisome): 0.1
Amino Acid Composition Tendency for Peroxisome: 10.47
Peroxisomal proteins? Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
Score: -2.76 Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..
----- Final Results -----
endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
plasma membrane --- Certainty= 0.640(Affirmative) < succ>
Golgi body --- Certainty= 0.460(Affirmative) < succ>
endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
----- The End -----