Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFMFLIF PFQFIPTSIF ILGFRLNSQQ TDKISSIICK YFFYLSYLVI
 KFISNMQCTH EMFIFKKIWF VFLKFHLKVN IHYLIILGGS NVFFFYHELL
 HVKKYFIYSP RRGSIHYFLK FTNISISILP LILFCFYLFL EDGGFQSITI
 HGHFTLISII IPQNQISGVT NPTPLKIISS SRFVRKRIQV WFRILVSHWV
 LEIKVLFKVF LHNSTIWNIW ILYVPSIMS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 4
     Position of the most N-terminal TMS: 6 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 13   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   23
     Peak Value of UR:   3.02
     Net Charge of CR: 0
     Discriminant Score:      9.96
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.15
     Possible cleavage site: 17
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.76  threshold: -2.0
     INTEGRAL    Likelihood = -8.76   Transmembrane  124 - 140 ( 118 - 144)
     INTEGRAL    Likelihood = -3.40   Transmembrane    6 -  22 (   1 -  24)
     PERIPHERAL  Likelihood = -0.69
     modified ALOM score:   1.85
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 114
     RRGSIH
Discrimination of mitochondrial target seq.: 
     positive ( 1.92)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.72 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  4.86
Chloroplast protein?  Status: negative (-5.62)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 114)  from: 1  to: 24  Score: 12.0
>>> Seems to have an intramitochondrial signal
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(229), count: 0   
Amino Acid Composition Tendency for Peroxisome:  17.95
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  0.82  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----