Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFLWVRS LYCMSIYKQQ GRRQEGEEKD GKEEVLQEGW RYTYRARMGL
 RRKLHRLLLR RGRCQHRHQQ RSYFPQRRPQ VSHGIAPVLP GFPTPKEGGH
 LSSSSQSLEI QDSQGSQAPL EQIQTMHWCP KSCTAQSNSR GKMAKNLLSN
 SCLNPDSSAD SAVPVVWGQR HARISSAWRG RRCCSGRSTR TCPGSCRCLR
 RCRPWRCRCR GRRCS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          215 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   1.06
     Net Charge of CR: 1
     Discriminant Score:     -7.67
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.57
     Possible cleavage site: 13
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.51  threshold: -2.0
     PERIPHERAL  Likelihood =  4.51
     modified ALOM score:  -1.80
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     RRQEGE
Discrimination of mitochondrial target seq.: 
     positive ( 2.12)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.66 at (46)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  3.53
Chloroplast protein?  Status: negative (-9.97)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 25)  from: 1  to: 6  Score:  6.0
Mitochondrial matrix?  Score:  0.43
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(215), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.11
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.081
Amino acid composition tendency for vacuolar proteins
     Score: -13.63  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.608(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.312(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.312(Affirmative) < succ>
     mitochondrial outer membrane --- Certainty= 0.312(Affirmative) < succ>


----- The End -----