Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFKFRHH LLKLHKPGCN KVIDIMIRKH KRHSTSNGKT TITTTELISV
 HDLKNKHPLT RSFLNHDCVW AALLERWMCC YIPLPLMSNK HLHVMQEDET
 SQQANCSDEI FKPQIELINI LKESQSRSEI LVDIELSSPN ITNIISFPRT
 TTIGSITPPR VNNTCKYHFN VCIGMQCVGT LSSYLEYKTT SSANITSMNA
 YECNAYVLCL HTSKGMKPHR TPPRATYDAK LYLVP

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          235 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   1.09
     Net Charge of CR: 4
     Discriminant Score:     -7.32
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -8.7
     Possible cleavage site: 21
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   3.45  threshold: -2.0
     PERIPHERAL  Likelihood =  3.45
     modified ALOM score:  -1.59
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 34
     KRHSTS
Discrimination of mitochondrial target seq.: 
     negative (-2.66)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.09 at (57)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  0.50
Chloroplast protein?  Status: negative (-12.87)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 229(235), count: 1   AKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -1.87
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score:  0.17  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  27 (3) RKHK
     Found: pos:  28 (3) KHKR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.600(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----