Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFILFFF FFFCFFLQGP KPFFFTMTTQ TIKILLTAPN QTQILLIKVQ
 KSLRLRSAQN EFDKVRSVGK KIRAFQIVFK LPNRIYVSVV AKTNQRCFWW
 WISWPKRGRT QNSKGINDQQ LDTTDATKNS TSPNVVLVNA QWFIGLRNST
 IFWLFLDYRR ENSEEKISHR TLLLPTDVHP LGDCPIFREG VELDWGRRRR
 SRPDYSLPKT LRLSNRYTTS YGC

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          223 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 2 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 9   Charge diffirence(C-N):  1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   19
     Peak Value of UR:   3.14
     Net Charge of CR: 0
     Discriminant Score:      9.53
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.27
     Possible cleavage site: 19
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 20
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 1B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.77  threshold: -2.0
     PERIPHERAL  Likelihood =  4.77
     modified ALOM score:  -1.85
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 58
     LRSAQN
Discrimination of mitochondrial target seq.: 
     positive ( 1.94)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.10 at (46)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  0.95
Chloroplast protein?  Status: negative (-9.99)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Number of Potential N-glycosylation Sites: 3
Out: score 0.800
Checking apolar signal for intramitochondrial sorting
  (Gavel position 58)  from: 1  to: 17  Score: 16.5
>>> Seems to have an intramitochondrial signal
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(223), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.45
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.182
Amino acid composition tendency for vacuolar proteins
     Score: -11.79  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 196 (5) RRRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.04)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          outside --- Certainty= 0.820(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.182(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.100(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----