Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFGLYFL VTKCTQNKIQ HDAFGSIYFY LFLGMVFTYD DARSNSHKEK
 MFLYWYYFQI TKWVLHEVDE RLLFSALLHG QVPERLLTIV KHIELQKSSC
 KKFRHGLSSH LQKKSLVFLL ASSSTTLSSS KFVNNVEVQR IGIKIFLLEM
 HYQVTKNNLS CASTCYNEGM ETLACCISSL LQIFDGTSDT ETLTSLVATQ
 STDIHRSSKM AHTCSDQIMK REAIKNA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          227 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   2.31
     Net Charge of CR: 0
     Discriminant Score:      1.32
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.16
     Possible cleavage site: 38
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.01  threshold: -2.0
     PERIPHERAL  Likelihood =  1.01
     modified ALOM score:  -1.10
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
(39) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 39
     Uncleavable? Ipos set to: 49
Discrimination of mitochondrial target seq.: 
     notclr (-0.16)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.39 at (66)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  3.72
Chloroplast protein?  Status: negative (-8.92)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 49)  from: 1  to: 11  Score:  9.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 109(227), count: 1   SHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   5.47
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score: -1.90  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.550(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.388(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
                          outside --- Certainty= 0.100(Affirmative) < succ>


----- The End -----