Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFFMLTH NGQNIYYHHG DCVTTTTTTN QVTTLVQQWP YIMPTNYEEN
 LNLSYISTTA TWGSVLIESF CMNSAHPQQL LIKPNLQMQE NLIVLAYFGQ
 QKEGQVSSPL LRNGEQSCPY EQYPRCLDQN KWSIVTASFI HSRRSRTTSL
 YLILRPSRCW VRTSKQVHKL WGCLLKHTNT VGSLNVDSPT TRVATIHARC
 NTRHRQSSRA YTSPNIAPTQ N

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          221 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   0.00
     Net Charge of CR: 1
     Discriminant Score:    -15.40
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.7
     Possible cleavage site: 14
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   5.41  threshold: -2.0
     PERIPHERAL  Likelihood =  5.41
     modified ALOM score:  -1.98
(47) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 47
     Uncleavable? Ipos set to: 57
Discrimination of mitochondrial target seq.: 
     negative (-4.54)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.74 at (44)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.01
     score from the 1-31 region:  1.98
Chloroplast protein?  Status: negative (-9.27)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(221), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.80
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.306
Amino acid composition tendency for vacuolar proteins
     Score: -12.41  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.306(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----