Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFFFFLS RGSVTPPRCP TSSLRRPQRA GSLSTLILPL PTTKVKLTHS
 LLKRAGNTNF LVVFHKIWRL TRNTSSRSKL QEIHKELDVV PKLERRRARE
 IRSTKPQLSS SLKDFSPKIN GRGKKCVKKR GRCFLGKWSS NRAHYFGERG
 RRYIWELKTS RWAKFSAGRL SLPLRRLNLP WQVR

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          184 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   6
     Peak Value of UR:  -0.36
     Net Charge of CR: 1
     Discriminant Score:    -18.24
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.42
     Possible cleavage site: 15
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   6.63  threshold: -2.0
     PERIPHERAL  Likelihood =  6.63
     modified ALOM score:  -2.23
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 79
     SRSKLQ
Discrimination of mitochondrial target seq.: 
     notclr ( 1.34)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.15 at (37)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  7.14
Chloroplast protein?  Status: negative (-4.45)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 79)  from: 1  to: 9  Score:  9.0
Mitochondrial matrix?  Score:  0.36
Mitochondrial intermemb.space?  Score:  0.30
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 78(184), count: 1   SKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   7.04
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -6.79  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  23.4% ( 0.47)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
                          nucleus --- Certainty= 0.628(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.510(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.464(Affirmative) < succ>


----- The End -----