Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFFAQQD FIFFICTVPT NSRLFTENTW RQCYVWSQHK PHTHAFVWCQ
 KFEHIVNNRW LSKKIIIIIR KALSKGETEY LSTVQTNKTR VLLRREIKTP
 GELQTWREYR FPSYTALISR GALFRQRFLQ EELILGTLRH ICSVGQFLYR
 SSPQGAPLAS RKGSPVDNQM AGKRRESCVP QTASASRQAG KAAPLPIPWC
 LTSRTRRCRR RLHQSVHSGW FFL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          223 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 2 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 9   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   12
     Peak Value of UR:   2.92
     Net Charge of CR: -1
     Discriminant Score:      5.24
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.46
     Possible cleavage site: 24
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.96  threshold: -2.0
     PERIPHERAL  Likelihood = -0.96
     modified ALOM score:  -0.71
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 72
     IRKALS
Discrimination of mitochondrial target seq.: 
     notclr ( 1.22)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  5.03 at (46)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  3.40
Chloroplast protein?  Status: negative (-9.91)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 72)  from: 1  to: 7  Score:  7.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 22(223), count: 1   SRL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:   8.73
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score: -12.39  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 160 (3) RK GSPVDNQMAG KRRES
Final Robbins Score (nucleus):  0.18
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.08)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.550(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.388(Affirmative) < succ>
                          nucleus --- Certainty= 0.180(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----