Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFCTARF HLFYLMHSTH LKFQVIHRKH LEAMLCLVTA QASHTCIRLV
 SKVAHRKQMA QKNNNNYTKG AFKGGNIISI HSADQQNKGS ASKGDQDSWG
 ASNLEGVSIP IIHMSPDIKM IRSTIPTKVP SRRVDLTRHS PSHLLSRPVF
 VPLVLSARCL ATGEPEGFSS RPDGWEARIV CLAPDSFSKS TSWESSSPSD
 SVVPNFSNPP LQKTITSVCP LGLVLP

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          226 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   0.79
     Net Charge of CR: 2
     Discriminant Score:     -9.84
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.72
     Possible cleavage site: 45
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.06  threshold: -2.0
     PERIPHERAL  Likelihood =  1.06
     modified ALOM score:  -1.11
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 58
     HRKQMA
Discrimination of mitochondrial target seq.: 
     notclr ( 1.44)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.45 at (37)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.01
     score from the 1-31 region:  2.74
Chloroplast protein?  Status: negative (-6.87)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 58)  from: 1  to: 8  Score:  6.0
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 142(226), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   1.85
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.313
Amino acid composition tendency for vacuolar proteins
     Score: -1.30  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.519(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.516(Affirmative) < succ>
                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.244(Affirmative) < succ>


----- The End -----