Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFIVTKL KVKISRRYCR SKHQVFMTRL PYTSHKKAEA EPIVFHATLR
 SSSSPLDFIT RTSLSHLRHP YPPLQLQPCP RPQCVLVRCR IMLTCHSPAA
 SRWTPLTQGK WRHLLSRFPW LGSHHHWCLQ LPALLQLPQS RHPQLAQCMP
 WMQCS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          155 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:  -0.40
     Net Charge of CR: 2
     Discriminant Score:    -19.36
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.49
     Possible cleavage site: 55
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.61  threshold: -2.0
     PERIPHERAL  Likelihood =  4.61
     modified ALOM score:  -1.82
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 31
     TRLPYT
Discrimination of mitochondrial target seq.: 
     positive ( 4.49)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.47 at (66)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.96
     score from the 1-31 region:  3.41
Chloroplast protein?  Status: negative (-6.97)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 31)  from: 1  to: 7  Score:  7.0
Mitochondrial matrix?  Score:  0.70
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 65(155), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   6.31
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -10.56  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.863(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.549(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.549(Affirmative) < succ>


----- The End -----