Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFISTSSP LKIATGMQQS INRYHDKKTK TLIIYQWEDN HNNMNINQCA
 RLVKKQTSFD KKFLIKSLCL GSIIMRVKVD VLLHPTPLDE QQAPALSNAR
 VRNISASLFD ITTNINQHFK RITIKIRNTC RYRTSQYNQY HLISSNNHNR
 FYNTSPRQQY LQISLQCMHR NAMCRYSIFI SLRVNHIICK YHFNECIMQC
 ICPLPSYLGY EAASYPSSGN VRCIVPRA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          228 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 67 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 74   Charge diffirence(C-N): -4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   10
     Peak Value of UR:   1.67
     Net Charge of CR: 0
     Discriminant Score:     -3.44
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.54
     Possible cleavage site: 14
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.22  threshold: -2.0
     PERIPHERAL  Likelihood = -0.22
     modified ALOM score:  -0.86
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     NRYHDK
Discrimination of mitochondrial target seq.: 
     notclr ( 0.60)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.17 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.96
     score from the 1-31 region:  8.12
Chloroplast protein?  Status: negative (-1.22)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 25)  from: 1  to: 5  Score:  5.0
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 50(228), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -3.45
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -4.56  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.462(Affirmative) < succ>
                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.160(Affirmative) < succ>


----- The End -----