Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FFFFFHFKFM LHFKLRVKYR VKYTNEKINI NANMCIYICL YYLLYLITQM
 FSNMNTAHIL FIPREHHFKC IDNLNVYSKF PLLLIHFKRC YFQISIGRYF
 SLISFANPKI RILGVINSTP LNRNLVLEIC KKRDIISLVN FSFSFGFGNQ
 SFVSFLYGYL CLAIYYVRGT VMASIGVARK SIIRKDFINC GSFRWRFNLI
 FEDLSCLSSL MWLVFFGLSS W

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          221 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 35 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 42   Charge diffirence(C-N): -3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   0.61
     Net Charge of CR: 2
     Discriminant Score:    -11.67
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.12
     Possible cleavage site: 52
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.12  threshold: -2.0
     PERIPHERAL  Likelihood = -1.12
     modified ALOM score:  -0.68
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 22
     YRVKYT
Discrimination of mitochondrial target seq.: 
     positive ( 1.52)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.73 at (26)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  1.01
Chloroplast protein?  Status: negative (-10.70)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 22)  from: 34  to: 47  Score:  6.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(221), count: 0   
Amino Acid Composition Tendency for Peroxisome:  10.31
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  4.28  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.523(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.252(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.252(Affirmative) < succ>


----- The End -----