Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ETISWKIRCP SGRFSNETTS EKDKERDVVH SRGLTEDWVS YATSVHVQKK
 DNLKDLRKTN ETKKFRIVFF MRNPLRISKR ALLYAIVTVR ILLLDFLCIF
 LFLNILDPKT SGSIKVARIN LSCKLSRCLA SLYLRICSRS KILVLDERFW
 YYASNGALRV HDVVAWLINH SQTLRVVFPI NFLFSSMKGS SDIAAIAGSP
 VSFWLGVLCQ 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          210 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 86 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 93   Charge diffirence(C-N): -5.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:  -0.79
     Net Charge of CR: 1
     Discriminant Score:    -21.82
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.98
     Possible cleavage site: 44
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.60  threshold: -2.0
     INTEGRAL    Likelihood = -8.60   Transmembrane   86 - 102 (  80 - 108)
     INTEGRAL    Likelihood = -2.07   Transmembrane  193 - 209 ( 193 - 209)
     PERIPHERAL  Likelihood =  3.71
     modified ALOM score:   1.82
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 15
     GRFSNE
Discrimination of mitochondrial target seq.: 
     notclr ( 0.31)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.73 at (67)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.90
     score from the 1-31 region:  5.55
Chloroplast protein?  Status: negative (-2.54)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 15)  from: 67  to: 71  Score:  4.5
Mitochondrial inner membrane?  Score:  0.73
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.364
SKL motif (signal for peroxisomal protein): 
     pos: -1(210), count: 0   
Amino Acid Composition Tendency for Peroxisome:   2.40
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -1.76  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.763(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.364(Affirmative) < succ>


----- The End -----