Query Information

ORIGIN plant
BEGIN
>MYSEQ
 EHQRRPLHRH RHGRHRHRRQ RQLQPGHQVR VLRPEQHHRL PRHQAEDIRA
 QCRWPHTTGT AESYALDQSG FKQELLNRFF AIFPREFDCY AVHDFGHQCI
 VWICSKGAWE PCESCISRLW LELLKWPPSL GVGKPGKTGA IPDTCGRRWG
 KDLCWRCWQR PRRRRSRWSF RRSPILALYV YRHPSCNTSS FPSFSSPSCR
 RPCHCHLYMD IQSDRTYHKK KKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          224 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:  -0.96
     Net Charge of CR: 6
     Discriminant Score:    -20.92
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.76
     Possible cleavage site: 60
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   7.53  threshold: -2.0
     PERIPHERAL  Likelihood =  7.53
     modified ALOM score:  -2.41
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 35
     LRPEQH
Discrimination of mitochondrial target seq.: 
     negative (-2.53)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.20 at (46)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -2.77
     score from the 1-31 region:  2.37
Chloroplast protein?  Status: negative (-12.55)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 117(224), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   5.78
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -11.43  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.1% ( 0.30)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  15 (3) RHRR
     Found: pos: 159 (4) RPRR
     Found: pos: 160 (4) PRRR
     Found: pos: 161 (5) RRRR
     Found: pos: 217 (3) HKKK
     Found: pos: 218 (5) KKKK
     Found: pos: 219 (5) KKKK
     Found: pos: 220 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:   3 (3) RR PLHRHRHGRH RHRRQ
     Found:  pos: 146 (3) RR WGKDLCWRCW QRPRR
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.986(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----