Query Information

ORIGIN plant
BEGIN
>MYSEQ
 DTERLHKSNH STSIAHCDTI KLQPYSINRQ SKQRNDTQSL CITWRPSAAA
 EPRLLTCVPR RCTNPRTRCM LSYSRPLHLF SPSPVPSSGF PGGCARVLAR
 AELRLHLFHR AHRLGRRKPS PLALNSACRS LHGYGRAQIS RSVRSRRSVI
 AHIHLPPEGR LSPCPRTIPC CRL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          173 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   0.64
     Net Charge of CR: 2
     Discriminant Score:    -10.89
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.29
     Possible cleavage site: 50
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   9.44  threshold: -2.0
     PERIPHERAL  Likelihood =  9.44
     modified ALOM score:  -2.79
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     
Discrimination of mitochondrial target seq.: 
     negative (-2.56)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.99 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  8.62
Chloroplast protein?  Status: negative (-4.78)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(173), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -6.15
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -4.86  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 115 (4) RRKP
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----