Query Information

ORIGIN plant
BEGIN
>MYSEQ
 DRELPAKMKL DFQQQQCHNL SYWRITRSLW GIQPLRFGYD SKSTMLTKQL
 NHALVMLHSR RNTRIFTHRE QVSLTINISF TNTSQGIWIT YKISLCVPPI
 IYCLIKSLDQ VCLEKGICTG NQARGCGQRS RKGGPAYYGE GSAKRQSTSS
 FRWFFGLLNY LFFEHERKRR TPNPRSNLYF NEPRRVLYLS HSSFRWKTYP
 KANTYFFTKK WS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          212 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 89 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 96   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   6
     Peak Value of UR:  -0.43
     Net Charge of CR: 0
     Discriminant Score:    -19.35
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.58
     Possible cleavage site: 31
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.64  threshold: -2.0
     PERIPHERAL  Likelihood = -0.64
     modified ALOM score:  -0.77
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     
Discrimination of mitochondrial target seq.: 
     negative (-5.10)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 14.49 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  4.49
Chloroplast protein?  Status: negative (-6.16)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(212), count: 0   
Amino Acid Composition Tendency for Peroxisome:   6.34
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -8.42  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 166 (5) RKRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                          nucleus --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----