Query Information

ORIGIN plant
BEGIN
>MYSEQ
 CPPKGVRGRC RCPGRTRLLL TLEDHRKKPG TRACRERRTR GGRASTVSPG
 HSMISTRGGY QAEKPGQVLY SPNVPIASAS RAPAPSSAGE AVTDLKSAFA
 VRIDFCERLA PGIKKKTSAK FHFRSVNKPK KETFFARRRT MLRFNKEFSV
 EPVNHASWLD AWGRGLVVPD SAAMQWKGRS LNRPPSFFFK KKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          195 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   0.72
     Net Charge of CR: 4
     Discriminant Score:     -9.55
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -7.25
     Possible cleavage site: 58
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   8.06  threshold: -2.0
     PERIPHERAL  Likelihood =  8.06
     modified ALOM score:  -2.51
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 19
     TRLLLT
Discrimination of mitochondrial target seq.: 
     notclr ( 0.02)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.84 at (69)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.08
     score from the 1-31 region:  0.00
Chloroplast protein?  Status: negative (-11.44)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 19)  from: 18  to: 22  Score:  3.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(195), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.97
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.330
Amino acid composition tendency for vacuolar proteins
     Score: -3.17  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  22.6% ( 0.43)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  24 (3) HRKK
     Found: pos:  25 (4) RKKP
     Found: pos: 127 (4) KPKK
     Found: pos: 189 (5) KKKK
     Found: pos: 190 (5) KKKK
     Found: pos: 191 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 114 (3) KK TSAKFHFRSV NKPKK
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.977(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.425(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.330(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.102(Affirmative) < succ>


----- The End -----