Query Information

ORIGIN plant
BEGIN
>MYSEQ
 CLSVRTCSMD DRPGKQFNHE GGMGCPLNNR IQGVVHLVVV PSMGLGVGSL
 CQVWVRPLGR SAVHLGNLTG GYSPGESLRL RSDALLGHLG SDQWRVMISG
 QNGNHGLWVK CTTSAECLKT DISAVLTVMS GQGSSSDWYL IRDIMVQVAM
 RSMVLVMTMV LVSGILSVWK GYIGLITWVN AKTWLSISKI IPTKTTALIP
 TSS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          203 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 34 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 41   Charge diffirence(C-N):  1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:   0.24
     Net Charge of CR: -1
     Discriminant Score:    -17.21
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.17
     Possible cleavage site: 46
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -6.48  threshold: -2.0
     INTEGRAL    Likelihood = -6.48   Transmembrane  152 - 168 ( 144 - 171)
     INTEGRAL    Likelihood = -2.13   Transmembrane   34 -  50 (  34 -  51)
     PERIPHERAL  Likelihood =  6.05
     modified ALOM score:   1.40
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 7
     VRTCSM
Discrimination of mitochondrial target seq.: 
     negative (-2.88)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.35 at (37)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.01
     score from the 1-31 region:  4.92
Chloroplast protein?  Status: negative (-4.45)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.279
SKL motif (signal for peroxisomal protein): 
     pos: -1(203), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.81
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -6.00  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.279(Affirmative) < succ>


----- The End -----